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Transcriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells.

TitleTranscriptionally and Functionally Distinct Mesenchymal Subpopulations Are Generated from Human Pluripotent Stem Cells.
Publication TypeJournal Article
Year of Publication2018
AuthorsChin CJia, Li S, Corselli M, Casero D, Zhu Y, Bin He C, Hardy R, Péault B, Crooks GM
JournalStem Cell Reports
Volume10
Issue2
Pagination436-446
Date Published2018 Feb 13
ISSN2213-6711
Abstract

Various mesenchymal cell types have been identified as critical components of the hematopoietic stem/progenitor cell (HSPC) niche. Although several groups have described the generation of mesenchyme from human pluripotent stem cells (hPSCs), the capacity of such cells to support hematopoiesis has not been reported. Here, we demonstrate that distinct mesenchymal subpopulations co-emerge from mesoderm during hPSC differentiation. Despite co-expression of common mesenchymal markers (CD73, CD105, CD90, and PDGFRβ), a subset of cells defined as CD146CD73 expressed genes associated with the HSPC niche and supported the maintenance of functional HSPCs ex vivo, while CD146CD73 cells supported differentiation. Stromal support of HSPCs was contact dependent and mediated in part through high JAG1 expression and low WNT signaling. Molecular profiling revealed significant transcriptional similarity between hPSC-derived CD146 and primary human CD146 perivascular cells. The derivation of functionally diverse types of mesenchyme from hPSCs opens potential avenues to model the HSPC niche and develop PSC-based therapies.

DOI10.1016/j.stemcr.2017.12.005
Alternate JournalStem Cell Reports
PubMed ID29307583
PubMed Central IDPMC5830911
Grant ListG1000816 / / Medical Research Council / United Kingdom
P30 AI028697 / AI / NIAID NIH HHS / United States
P30 CA016042 / CA / NCI NIH HHS / United States
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