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Semi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells.

TitleSemi-automated Production of Hepatocyte Like Cells from Pluripotent Stem Cells.
Publication TypeJournal Article
Year of Publication2018
AuthorsMeseguer-Ripolles J, Lucendo-Villarin B, Wang Y, Hay DC
JournalJ Vis Exp
Issue137
Date Published2018 07 27
ISSN1940-087X
Abstract

Human pluripotent stem cells represent a renewable source of human tissue. Our research is focused on generating human liver tissue from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). Current differentiation procedures generate human hepatocyte-like cells (HLCs) displaying a mixture of fetal and adult traits. To improve cell phenotype, we have fully defined our differentiation procedure and the cell niche, resulting in the generation of cell populations which display improved gene expression and function. While these studies mark progress, the ability to generate large quantities of multi well plates for screening has been limited by labour intensive procedures and batch to batch variation. To tackle this issue, we have developed a semi-automated platform to differentiate pluripotent stem cells into HLCs. Stem cell seeding and differentiation were performed using liquid handling and automatic pipetting systems in 96-well plate format. Following the differentiation, cell phenotype was analyzed using automated microscopy and a multi well luminometer.

DOI10.3791/57995
Alternate JournalJ Vis Exp
PubMed ID30102283
Grant ListTCS/16/37 / / Chief Scientist Office / United Kingdom
/ / Medical Research Council / United Kingdom
Publication institute
CRM