|Title||[Reconstruction of the organotypic structure of embryonic lung tissue from a suspension of dissociated cells].|
|Publication Type||Journal Article|
|Year of Publication||1982|
|Authors||Kolesnichenko TS, Medvinskiĭ AL|
|Keywords||Animals, Cells, Cultured, Chick Embryo, Lung, Morphogenesis, Suspensions, Time Factors|
The formation and development of organotypic structure of the embryonic lung tissue was studied upon the cultivation of a suspension of dissociated cells of the lungs of 8-11 days old chick embryos as a hanging drop. The successive developmental stages of organotypic structures are discussed: 1) passive sedimentation of dissociated cells on the "bottom" of hanging drop; 2) redistribution of sedimented cells on the tension surface of the drop with the formation of multiple cell aggregations (centers of aggregation) connected by monolayer mesenchyme constrictions; 3) formation of a primary aggregate as a flat celled structure; 4) formation of a space organotypic aggregate with the structure characteristic for the initial lung tissue.