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[Reconstruction of the organotypic structure of embryonic lung tissue from a suspension of dissociated cells].

Title[Reconstruction of the organotypic structure of embryonic lung tissue from a suspension of dissociated cells].
Publication TypeJournal Article
Year of Publication1982
AuthorsKolesnichenko TS, Medvinskiĭ AL
JournalOntogenez
Volume13
Issue1
Pagination83-7
Date Published1982
ISSN0475-1450
KeywordsAnimals, Cells, Cultured, Chick Embryo, Lung, Morphogenesis, Suspensions, Time Factors
Abstract

The formation and development of organotypic structure of the embryonic lung tissue was studied upon the cultivation of a suspension of dissociated cells of the lungs of 8-11 days old chick embryos as a hanging drop. The successive developmental stages of organotypic structures are discussed: 1) passive sedimentation of dissociated cells on the "bottom" of hanging drop; 2) redistribution of sedimented cells on the tension surface of the drop with the formation of multiple cell aggregations (centers of aggregation) connected by monolayer mesenchyme constrictions; 3) formation of a primary aggregate as a flat celled structure; 4) formation of a space organotypic aggregate with the structure characteristic for the initial lung tissue.

Alternate JournalOntogenez
PubMed ID7063168
Publication institute
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