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Real-time label-free monitoring of adipose-derived stem cell differentiation with electric cell-substrate impedance sensing.

TitleReal-time label-free monitoring of adipose-derived stem cell differentiation with electric cell-substrate impedance sensing.
Publication TypeJournal Article
Year of Publication2011
AuthorsBagnaninchi PO, Drummond N
JournalProc Natl Acad Sci U S A
Volume108
Issue16
Pagination6462-7
Date Published2011 Apr 19
ISSN1091-6490
KeywordsAdipose Tissue, Cell Differentiation, Cells, Cultured, Electric Impedance, Humans, Osteogenesis, Stem Cells
Abstract

Real-time monitoring of stem cells (SCs) differentiation will be critical to scale-up SC technologies, while label-free techniques will be desirable to quality-control SCs without precluding their therapeutic potential. We cultured adipose-derived stem cells (ADSCs) on top of multielectrode arrays and measured variations in the complex impedance Z* throughout induction of ADSCs toward osteoblasts and adipocytes. Z* was measured up to 17 d, every 180 s, over a 62.5-64 kHz frequency range with an ECIS Z instrument. We found that osteogenesis and adipogenesis were characterized by distinct Z* time-courses. Significant differences were found (P = 0.007) as soon as 12 h post induction. An increase in the barrier resistance (Rb) up to 1.7 ohm·cm(2) was associated with early osteo-induction, whereas Rb peaked at 0.63 ohm·cm(2) for adipo-induced cells before falling to zero at t = 129 h. Dissimilarities in Z* throughout early induction (

DOI10.1073/pnas.1018260108
Alternate JournalProc. Natl. Acad. Sci. U.S.A.
PubMed ID21464296
PubMed Central IDPMC3080969
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