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Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair.

TitlePerivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair.
Publication TypeJournal Article
Year of Publication2016
AuthorsHindle P, Baily J, Khan N, Biant LC, A Simpson HR, Péault B
JournalStem Cells Dev
Volume25
Issue21
Pagination1659-1669
Date Published2016 11 01
ISSN1557-8534
KeywordsAnimals, Antibodies, Blood Vessels, Cartilage, Articular, Cell Differentiation, Cell Proliferation, Cells, Cultured, Cross Reactions, Disease Models, Animal, Flow Cytometry, Green Fluorescent Proteins, Hydrogel, Polyethylene Glycol Dimethacrylate, Immunohistochemistry, Mesenchymal Stem Cell Transplantation, Mesenchymal Stromal Cells, Pericytes, Sheep, Transfection, Transplantation, Autologous, Wound Healing
Abstract

Previous research has indicated that purified perivascular stem cells (PSCs) have increased chondrogenic potential compared to conventional mesenchymal stem cells (MSCs) derived in culture. This study aimed to develop an autologous large animal model for PSC transplantation and to specifically determine if implanted cells are retained in articular cartilage defects. Immunohistochemistry and fluorescence-activated cell sorting were used to ascertain the reactivity of anti-human and anti-ovine antibodies, which were combined and used to identify and isolate pericytes (CD34-CD45-CD146+) and adventitial cells (CD34+CD45-CD146-). The purified cells demonstrated osteogenic, adipogenic, and chondrogenic potential in culture. Autologous ovine PSCs (oPSCs) were isolated, cultured, and efficiently transfected using a green fluorescence protein (GFP) encoding lentivirus. The cells were implanted into articular cartilage defects on the medial femoral condyle using hydrogel and collagen membranes. Four weeks following implantation, the condyle was explanted and confocal laser scanning microscopy demonstrated the presence of oPSCs in the defect repaired with the hydrogel. These data suggest the testability in a large animal of native MSC autologous grafting, thus avoiding possible biases associated with xenotransplantation. Such a setting will be used in priority for indications in orthopedics, at first to model articular cartilage repair.

DOI10.1089/scd.2016.0165
Alternate JournalStem Cells Dev.
PubMed ID27554322
Grant ListMR/K015710/1 / / Medical Research Council / United Kingdom
MR/L012766/1 / / Medical Research Council / United Kingdom
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