Leading science, pioneering therapies
CRM Publications

Perivascular cells for regenerative medicine.

TitlePerivascular cells for regenerative medicine.
Publication TypeJournal Article
Year of Publication2012
AuthorsCrisan M, Corselli M, Chen WCW, Péault B
JournalJ Cell Mol Med
Volume16
Issue12
Pagination2851-60
Date Published2012 Dec
ISSN1582-4934
KeywordsAdipose Tissue, Adventitia, Antigens, Antigens, CD146, Biomarkers, Cell Differentiation, Cell Lineage, Cell Transplantation, Cells, Cultured, Humans, Membrane Proteins, Mesenchymal Stromal Cells, Pericytes, Proteoglycans, Receptor, Platelet-Derived Growth Factor beta, Regenerative Medicine
Abstract

Mesenchymal stem/stromal cells (MSC) are currently the best candidate therapeutic cells for regenerative medicine related to osteoarticular, muscular, vascular and inflammatory diseases, although these cells remain heterogeneous and necessitate a better biological characterization. We and others recently described that MSC originate from two types of perivascular cells, namely pericytes and adventitial cells and contain the in situ counterpart of MSC in developing and adult human organs, which can be prospectively purified using well defined cell surface markers. Pericytes encircle endothelial cells of capillaries and microvessels and express the adhesion molecule CD146 and the PDGFRβ, but lack endothelial and haematopoietic markers such as CD34, CD31, vWF (von Willebrand factor), the ligand for Ulex europaeus 1 (UEA1) and CD45 respectively. The proteoglycan NG2 is a pericyte marker exclusively associated with the arterial system. Besides its expression in smooth muscle cells, smooth muscle actin (αSMA) is also detected in subsets of pericytes. Adventitial cells surround the largest vessels and, opposite to pericytes, are not closely associated to endothelial cells. Adventitial cells express CD34 and lack αSMA and all endothelial and haematopoietic cell markers, as for pericytes. Altogether, pericytes and adventitial perivascular cells express in situ and in culture markers of MSC and display capacities to differentiate towards osteogenic, adipogenic and chondrogenic cell lineages. Importantly, adventitial cells can differentiate into pericyte-like cells under inductive conditions in vitro. Altogether, using purified perivascular cells instead of MSC may bring higher benefits to regenerative medicine, including the possibility, for the first time, to use these cells uncultured.

DOI10.1111/j.1582-4934.2012.01617.x
Alternate JournalJ. Cell. Mol. Med.
PubMed ID22882758
PubMed Central IDPMC4393715
Grant ListMRC_G1000816 / / Medical Research Council / United Kingdom
/ / Medical Research Council / United Kingdom
Publication institute
Other