Leading science, pioneering therapies
CRM Publications

The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines.

TitleThe Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines.
Publication TypeJournal Article
Year of Publication2015
AuthorsCanham MA, Van Deusen A, Brison DR, De Sousa PA, Downie J, Devito L, Hewitt ZA, Ilic D, Kimber SJ, Moore HD, Murray H, Kunath T
JournalSci Rep
Volume5
Pagination17258
Date Published2015 Nov 26
ISSN2045-2322
KeywordsCell Line, Databases, Genetic, Gene Deletion, Gene Duplication, Human Embryonic Stem Cells, Humans, Karyotyping, Polymorphism, Single Nucleotide
Abstract

The application of human embryonic stem cell (hESC) derivatives to regenerative medicine is now becoming a reality. Although the vast majority of hESC lines have been derived for research purposes only, about 50 lines have been established under Good Manufacturing Practice (GMP) conditions. Cell types differentiated from these designated lines may be used as a cell therapy to treat macular degeneration, Parkinson's, Huntington's, diabetes, osteoarthritis and other degenerative conditions. It is essential to know the genetic stability of the hESC lines before progressing to clinical trials. We evaluated the molecular karyotype of 25 clinical-grade hESC lines by whole-genome single nucleotide polymorphism (SNP) array analysis. A total of 15 unique copy number variations (CNVs) greater than 100 kb were detected, most of which were found to be naturally occurring in the human population and none were associated with culture adaptation. In addition, three copy-neutral loss of heterozygosity (CN-LOH) regions greater than 1 Mb were observed and all were relatively small and interstitial suggesting they did not arise in culture. The large number of available clinical-grade hESC lines with defined molecular karyotypes provides a substantial starting platform from which the development of pre-clinical and clinical trials in regenerative medicine can be realised.

DOI10.1038/srep17258
Alternate JournalSci Rep
PubMed ID26607962
PubMed Central IDPMC4660465
Grant ListG0700092 / / Medical Research Council / United Kingdom
G0801061 / / Medical Research Council / United Kingdom
G0801059 / / Medical Research Council / United Kingdom
K-1205 / / Parkinson's UK / United Kingdom
G0300484 / / Medical Research Council / United Kingdom
G0801057 / / Medical Research Council / United Kingdom
MR/M017354/1 / / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom
MR/L020335/1 / / Medical Research Council / United Kingdom
MR/K017276/1 / / Medical Research Council / United Kingdom
MR/L004992/1 / / Medical Research Council / United Kingdom
Publication institute
CRM