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Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging.

TitleMapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging.
Publication TypeJournal Article
Year of Publication2019
AuthorsWisniewski D, Lowell S, Blin G
JournalJ Vis Exp
Issue146
Date Published2019 Apr 30
ISSN1940-087X
Abstract

A fundamental goal in biology is to understand how patterns emerge during development. Several groups have shown that patterning can be achieved in vitro when stem cells are spatially confined onto micropatterns, thus setting up experimental models which offer unique opportunities to identify, in vitro, the fundamental principles of biological organisation. Here we describe our own implementation of the methodology. We adapted a photo-patterning technique to reduce the need for specialized equipment to make it easier to establish the method in a standard cell biology laboratory. We also developed a free, open-source and easy to install image analysis framework in order to precisely measure the preferential positioning of sub-populations of cells within colonies of standard shapes and sizes. This method makes it possible to reveal the existence of patterning events even in seemingly disorganized populations of cells. The technique provides quantitative insights and can be used to decouple influences of the environment (e.g., physical cues or endogenous signaling), on a given patterning process.

DOI10.3791/59634
Alternate JournalJ Vis Exp
PubMed ID31107437
Publication institute
CRM