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Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.

TitleIsolation of blood-vessel-derived multipotent precursors from human skeletal muscle.
Publication TypeJournal Article
Year of Publication2014
AuthorsChen WCW, Saparov A, Corselli M, Crisan M, Zheng B, Péault B, Huard J
JournalJ Vis Exp
Issue90
Paginatione51195
Date Published2014
ISSN1940-087X
Abstract

Since the discovery of mesenchymal stem/stromal cells (MSCs), the native identity and localization of MSCs have been obscured by their retrospective isolation in culture. Recently, using fluorescence-activated cell sorting (FACS), we and other researchers prospectively identified and purified three subpopulations of multipotent precursor cells associated with the vasculature of human skeletal muscle. These three cell populations: myogenic endothelial cells (MECs), pericytes (PCs), and adventitial cells (ACs), are localized respectively to the three structural layers of blood vessels: intima, media, and adventitia. All of these human blood-vessel-derived stem cell (hBVSC) populations not only express classic MSC markers but also possess mesodermal developmental potentials similar to typical MSCs. Previously, MECs, PCs, and ACs have been isolated through distinct protocols and subsequently characterized in separate studies. The current isolation protocol, through modifications to the isolation process and adjustments in the selective cell surface markers, allows us to simultaneously purify all three hBVSC subpopulations by FACS from a single human muscle biopsy. This new method will not only streamline the isolation of multiple BVSC subpopulations but also facilitate future clinical applications of hBVSCs for distinct therapeutic purposes.

DOI10.3791/51195
Alternate JournalJ Vis Exp
PubMed ID25177794
Publication institute
CRM