Leading science, pioneering therapies
CRM Publications

Human kidney pericytes produce renin.

TitleHuman kidney pericytes produce renin.
Publication TypeJournal Article
Year of Publication2016
AuthorsStefanska A, Kenyon C, Christian HC, Buckley C, Shaw I, Mullins JJ, Péault B
JournalKidney Int
Date Published2016 Sep 24
ISSN1523-1755
Abstract

Pericytes, perivascular cells embedded in the microvascular wall, are crucial for vascular homeostasis. These cells also play diverse roles in tissue development and regeneration as multi-lineage progenitors, immunomodulatory cells and as sources of trophic factors. Here, we establish that pericytes are renin producing cells in the human kidney. Renin was localized by immunohistochemistry in CD146 and NG2 expressing pericytes, surrounding juxtaglomerular and afferent arterioles. Similar to pericytes from other organs, CD146(+)CD34(-)CD45(-)CD56(-) renal fetal pericytes, sorted by flow cytometry, exhibited tri-lineage mesodermal differentiation potential in vitro. Additionally, renin expression was triggered in cultured kidney pericytes by cyclic AMP as confirmed by immuno-electron microscopy, and secretion of enzymatically functional renin, capable of generating angiotensin I. Pericytes derived from second trimester human placenta also expressed renin in an inducible fashion although the renin activity was much lower than in renal pericytes. Thus, our results confirm and extend the recently discovered developmental plasticity of microvascular pericytes, and may open new perspectives to the therapeutic regulation of the renin-angiotensin system.

DOI10.1016/j.kint.2016.07.035
Alternate JournalKidney Int.
PubMed ID27678158
Publication institute
CRM