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Developing defined substrates for stem cell culture and differentiation.

TitleDeveloping defined substrates for stem cell culture and differentiation.
Publication TypeJournal Article
Year of Publication2018
AuthorsHagbard L, Cameron K, August P, Penton C, Parmar M, Hay DC, Kallur T
JournalPhilos Trans R Soc Lond B Biol Sci
Volume373
Issue1750
Date Published2018 Jul 05
ISSN1471-2970
Abstract

Over the past few decades, a variety of different reagents for stem cell maintenance and differentiation have been commercialized. These reagents share a common goal in facilitating the manufacture of products suitable for cell therapy while reducing the amount of non-defined components. Lessons from developmental biology have identified signalling molecules that can guide the differentiation process , but less attention has been paid to the extracellular matrix used. With the introduction of more biologically relevant and defined matrices, that better mimic specific cell niches, researchers now have powerful resources to fine-tune their differentiation systems, which may allow the manufacture of therapeutically relevant cell types. In this review article, we revisit the basics of the extracellular matrix, and explore the important role of the cell-matrix interaction. We focus on laminin proteins because they help to maintain pluripotency and drive cell fate specification.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.

DOI10.1098/rstb.2017.0230
Alternate JournalPhilos. Trans. R. Soc. Lond., B, Biol. Sci.
PubMed ID29786564
PubMed Central IDPMC5974452
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CRM