|Title||Complementary tissue-specific expression of LIF and LIF-receptor mRNAs in early mouse embryogenesis.|
|Publication Type||Journal Article|
|Year of Publication||1996|
|Authors||Nichols J, Davidson D, Taga T, Yoshida K, Chambers I, Smith A|
|Date Published||1996 Jul|
|Keywords||Animals, Embryonic and Fetal Development, Female, Growth Inhibitors, Interleukin-6, Leukemia Inhibitory Factor, Leukemia Inhibitory Factor Receptor alpha Subunit, Lymphokines, Male, Mice, Morpholines, Organ Specificity, Pregnancy, Receptors, Cytokine, Receptors, OSM-LIF, RNA, Messenger, Stem Cells|
The maintenance of pluripotential embryonic stem (ES) cells is dependent on the cytokine LIF. This report documents the mRNA expression profiles of LIF and the two components of the LIF-receptor complex, LIF-R and gp130, during early mouse embryogenesis. These mRNAs were undetectable in 1- or 2-cell embryos, but all were present by the blastocyst stage. LIF transcripts were localised in the differentiated trophectoderm, and were absent from the pluripotential inner cell mass. In contrast, LIF-R mRNA was found in the inner cell mass but not in the trophectoderm. This complementary pattern of expression is suggestive of a paracrine coupling between stem cells and differentiated progeny at the earliest stage of mammalian development. After implantation, transcripts for all components were down-regulated in the embryo. High levels of LIF-R and gp130 mRNAs were observed in the deciduum, however. These dynamic, tissue-specific expression patterns are consistent with regulatory roles for LIF or related cytokines, both in the maintenance of pluripotency in the mouse embryo, and in development of the foeto-maternal interface.
|Alternate Journal||Mech. Dev.|