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Chitosan microchannel scaffolds for tendon tissue engineering characterized using optical coherence tomography.

TitleChitosan microchannel scaffolds for tendon tissue engineering characterized using optical coherence tomography.
Publication TypeJournal Article
Year of Publication2007
AuthorsBagnaninchi PO, Yang Y, Zghoul N, Maffulli N, Wang RK, Haj AJEl
JournalTissue Eng
Volume13
Issue2
Pagination323-31
Date Published2007 Feb
ISSN1076-3279
KeywordsAnimals, Biocompatible Materials, Cell Culture Techniques, Cells, Cultured, Chitosan, Swine, Tendons, Tissue Engineering, Tomography, Optical Coherence
Abstract

Tendon tissue engineering requires the generation of a uniaxially orientated collagen type I matrix with several organization scales that confer mechanical functionality upon the tendon. A combination of factors in a dose- and time-dependent manner, such as growth factors and mechanical environment, may be the key to an in vitro-engineered tendon. To define the progress of tissue development within a scaffold, on-line systems need to be applied to monitor the newly generated matrix. To address this challenge, we designed a new porous chitosan scaffold with microchannels (diameter: 250 microm), which allows primary porcine tenocytes to proliferate in a bundle-like structure. The cell proliferation and extracellular matrix (ECM) production within the microchannels were successfully assessed under sterile conditions using optical coherence tomography (OCT). A semi-quantitative method that calculated the microchannel occupation ratio (the degree of cell proliferation and tissue turnover based on the total backscattered intensity in the microchannels) was developed. We further investigated the effect of different culture conditions on tendon cell matrix formation. Using a perfusion bioreactor, we demonstrated how fluid flow can increase (p

DOI10.1089/ten.2006.0168
Alternate JournalTissue Eng.
PubMed ID17518566
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