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Cellular micromotion monitored by long-range surface plasmon resonance with optical fluctuation analysis.

TitleCellular micromotion monitored by long-range surface plasmon resonance with optical fluctuation analysis.
Publication TypeJournal Article
Year of Publication2015
AuthorsYang C-T, Méjard R, Griesser HJ, Bagnaninchi PO, Thierry B
JournalAnal Chem
Volume87
Issue3
Pagination1456-61
Date Published2015 Feb 3
ISSN1520-6882
Keywords3T3 Cells, Animals, Cell Adhesion, Cell Line, Tumor, Cell Movement, Humans, Mice, Surface Plasmon Resonance
Abstract

Long-range surface plasmon resonance (LRSPR) is a powerful biosensing technology due to a substantially larger probing depth into the medium and sensitivity, compared with conventional SPR. We demonstrate here that LRSPR can provide sensitive noninvasive measurement of the dynamic fluctuation of adherent cells, often referred to as the cellular micromotion. Proof of concept was achieved using confluent layers of 3T3 fibroblast cells and MDA-MB-231 cancer cells. The slope of the power spectral density (PSD) of the optical fluctuations was calculated to determine the micromotion index, and significant differences were measured between live and fixed cell layers. Furthermore, the performances of LRSPR and conventional surface plasmon resonance (cSPR) were compared with respect to micromotion monitoring. Our study showed that the micromotion index of cells measured by LRSPR sensors was higher than when measured with cSPR, suggesting a higher sensitivity of LRSPR to the micromotion of cells. To investigate further this finding, simulations were conducted to establish the relative sensitivities of LRSPR and cSPR to membrane fluctuations. Increased signal intensity was predicted for LRSPR in comparison to cSPR, suggesting that membrane fluctuations play a significant role in the optical micromotion measured in LRSPR. Analogous to cellular micromotion measured using impedance techniques, LRSPR micromotion has the potential to provide important biological information on the metabolic activity and viability of adherent cells.

DOI10.1021/ac5031978
Alternate JournalAnal. Chem.
PubMed ID25495915
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