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Biosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6.

TitleBiosynthesis of magnetic nanoparticles by human mesenchymal stem cells following transfection with the magnetotactic bacterial gene mms6.
Publication TypeJournal Article
Year of Publication2017
AuthorsElfick A, Rischitor G, Mouras R, Azfer A, Lungaro L, Uhlarz M, Herrmannsdörfer T, Lucocq J, Gamal W, Bagnaninchi P, Semple S, Salter DM
JournalSci Rep
Volume7
Pagination39755
Date Published2017 Jan 04
ISSN2045-2322
Abstract

The use of stem cells to support tissue repair is facilitated by loading of the therapeutic cells with magnetic nanoparticles (MNPs) enabling magnetic tracking and targeting. Current methods for magnetizing cells use artificial MNPs and have disadvantages of variable uptake, cellular cytotoxicity and loss of nanoparticles on cell division. Here we demonstrate a transgenic approach to magnetize human mesenchymal stem cells (MSCs). MSCs are genetically modified by transfection with the mms6 gene derived from Magnetospirillum magneticum AMB-1, a magnetotactic bacterium that synthesises single-magnetic domain crystals which are incorporated into magnetosomes. Following transfection of MSCs with the mms6 gene there is bio-assimilated synthesis of intracytoplasmic magnetic nanoparticles which can be imaged by MR and which have no deleterious effects on cell proliferation, migration or differentiation. The assimilation of magnetic nanoparticle synthesis into mammalian cells creates a real and compelling, cytocompatible, alternative to exogenous administration of MNPs.

DOI10.1038/srep39755
Alternate JournalSci Rep
PubMed ID28051139
PubMed Central IDPMC5209691
Publication institute
CRM