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Association between active genes occurs at nuclear speckles and is modulated by chromatin environment.

TitleAssociation between active genes occurs at nuclear speckles and is modulated by chromatin environment.
Publication TypeJournal Article
Year of Publication2008
AuthorsBrown JM, Green J, Neves RPires das, Wallace HAC, Smith AG, Hughes J, Gray N, Taylor S, Wood WG, Higgs DR, Iborra FJ, Buckle VJ
JournalJ Cell Biol
Volume182
Issue6
Pagination1083-97
Date Published2008 Sep 22
ISSN1540-8140
KeywordsAnimals, Anion Exchange Protein 1, Erythrocyte, Blood Cells, Cell Nucleus, Chromatin, Chromosomes, Erythropoiesis, Gene Expression Regulation, Globins, Humans, In Situ Hybridization, Fluorescence, Intranuclear Inclusion Bodies, Mice, Multigene Family, Transcription, Genetic
Abstract

Genes on different chromosomes can be spatially associated in the nucleus in several transcriptional and regulatory situations; however, the functional significance of such associations remains unclear. Using human erythropoiesis as a model, we show that five cotranscribed genes, which are found on four different chromosomes, associate with each other at significant but variable frequencies. Those genes most frequently in association lie in decondensed stretches of chromatin. By replacing the mouse alpha-globin gene cluster in situ with its human counterpart, we demonstrate a direct effect of the regional chromatin environment on the frequency of association, whereas nascent transcription from the human alpha-globin gene appears unaffected. We see no evidence that cotranscribed erythroid genes associate at shared transcription foci, but we do see stochastic clustering of active genes around common nuclear SC35-enriched speckles (hence the apparent nonrandom association between genes). Thus, association between active genes may result from their location on decondensed chromatin that enables clustering around common nuclear speckles.

DOI10.1083/jcb.200803174
Alternate JournalJ. Cell Biol.
PubMed ID18809724
PubMed Central IDPMC2542471
Grant ListMC_U137961143 / / Medical Research Council / United Kingdom
MC_U137961144 / / Medical Research Council / United Kingdom
MC_U137961145 / / Medical Research Council / United Kingdom
MC_U137973816 / / Medical Research Council / United Kingdom
/ / Medical Research Council / United Kingdom