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An all-in-one UniSam vector system for efficient gene activation.

TitleAn all-in-one UniSam vector system for efficient gene activation.
Publication TypeJournal Article
Year of Publication2017
AuthorsFidanza A, Lopez-Yrigoyen M, Romanò N, Jones R, A Taylor H, Forrester LM
JournalSci Rep
Volume7
Issue1
Pagination6394
Date Published2017 Jul 25
ISSN2045-2322
Abstract

We have generated a drug-free, all-in-one dCAS9-SAM vector that can activate endogenous gene expression with the potential to modify cell fate. We demonstrate that this strategy can be used in a number of cell lines and avoids exceptionally high levels of gene expression that are observed in standard transgenic approaches. Compared to the multi-plasmid system, this all-in-one vector activates gene expression to a comparable level but the reduced overall DNA content results in significantly higher viability of transfected cells. This allowed us to use the RUNX1C-GFP human embryonic stem cell reporter cell line to monitor gene activation in individual cells and to show that activation could occur at all stages of the cell cycle.

DOI10.1038/s41598-017-06468-6
Alternate JournalSci Rep
PubMed ID28743878
PubMed Central IDPMC5526871
Grant List / / Wellcome Trust / United Kingdom
Publication institute
CRM