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Widefield Zeiss observer (live imaging)

Widefield Zeiss observer (live imaging)

Introduction

Location: SCRM ground floor, room G.45

Access (after training): 24h, 7/7 days

Cost (SCRM users): £15 peak hours (8am-5pm, week days), £11.25 off-peak, time-lapse session £60

Specifications

Main features: Widefield microscope dedicated to live imaging and deconvolution

  • Inverted microscope
  • Zeiss Colibri7 LED light source ( LEDs: 385, 475, 555, 590, 630 nm)
  • Filter cubes: DAPI, GFP, DsRed, mPlum and Cy5
  • Monochrome Hamamatsu flash 4.0 v3 camera
  • Colour Zeiss MRc camera
  • Motorised XY stage (multi-positions, mosaic)
  • Motorised Z-focus
  • Pecon incubation chamber with temperature and CO2 controllers

Filter cubes:

  • DAPI (365/65, 395, 480/40)
  • GFP (470/40, 495, 525/50)
  • dsRed (550/25, 570, 605/70)
  • mPlum (587/25, 605, 647/70)
  • Cy5 (620/60, 660, 700/75)

Objectives

  • 5 x/0.1
  • 10x/0.45
  • LWD 20x/ 0.5 (for multi-well plates)
  • LWD 40x/0.75 (for multi-well plates)
  • Plan-Apo 20x/0.8 
  • Plan-Apo 40x/1 OIL
  • Plan-apo 63x/1.4 OIL

Sample carriers:

  • Slides or chambered-slides
  • Petri dishes (35 mm, 50 mm, 60 mm)
  • Multi-well plates

Contact