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Leica TCS SP8 Confocal and Digital LightSheet (DLS)

Leica TCS SP8 Confocal and Digital LightSheet (DLS)

Introduction

Location: SCRM ground floor, room G.43

Access (after training): 24/24h, 7/7 days

Cost (SCRM members): £44peak hours (8am-5pm, week days), £33 off-peak, time-lapse session £200

Specifications

Main features:

  • Inverted microscope
  • Solid stateLasers: 405, 488, 552 and 638 nm
  • Filter-free spectral detection 400-800 nm
  • 2 HyD detectors (45% QE @ 500 nm) and 1 PMT (30% QE @ 500 nm) for fluorescence imaging, 1 PMT for transmitted light
  • Fully motorised XY stage for multi-positions, tilling, …
  • galvanometer stage for Z-focus (range 1500 µm, minimum step size 20 nm)
  • Digital LightSheet module

Objectives (confocal)

  • Obj. HC PL APO 10x/0.40 CS2
  • Obj. HC PL APO 20x/0.75 CS2
  • Obj. HC PL APO 40x/1.30 Oil CS2
  • Obj. HC PL APO 63x/1.40 OIL CS2

Digital LightSheet Illumination objectives:

  • L 1.6x/0.05 DLS
  • Obj. HC PL FLUOTAR 2.5x/0.07
  • HC PL FLUOTAR 5x/0.15

Digital LightSheet TwinFlect mirror:

  • DLS TwinFlect 2.5mm
  • DLS TwinFlect 5mm
  • DLS TwinFlect 7.8mm W
  • DLS TwinFlect 7.8mm Gly

Digital LightSheet Collection objectives:

  • HC PL FLUOTAR 5x/0.15 IMM DLS
  • Obj. HC APO L10x/0.30 W DLS
  • Obj. HC FLUOTAR L25x/0.95 W DLS

Digital LightSheet Emission Filters:

  • Filter DLS BandPass 455­-495
  • Filter DLS BandPass 504­-545
  • Filter DLS BandPass 575­-635
  • Filter DLS 405/488/561/633 (BandPass 422-­474nm, 503-­545nm, 582­-615nm, 662­-750nm)
  • Filter DLS 405/488/552/638 (BandPass 420­-475nm, 500­-540nm, 565­-620nm, 655­-750nm)
  • Filter DLS 458/514 (BandPass 470­-500nm, 530-­650nm)
  • Filter DLS 488/561 (BandPass 504­-544nm, 578­-707nm

Sample carriers:

  • Slides or chambered-slides
  • Petri dishes (35 mm, 50 mm)
  •  Multi-well plates

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