Cell therapies aim to improve patient health by delivering cells with therapeutic potential. To provide physical support and to confine the treatment delivery vehicles (gels, bioscaffold) are used. There is currently no non-destructive way to assess the state of the cells (number, distribution, identity, and viability) within these vehicles. We investigate the use of non destructive technologies to assess the quality and safety in 3D bioscaffolds. These technologies address also the need to monitor in a time-lapse fashion “disease in a dish” models.
Our main research interest is the development of label-free quantitative technologies to map biophysical properties (mechanical, optical, flow, electrical) of cells and tissues. They also address the need for real-time monitoring of 3D (spheroids, organoids) in vitro disease models, and the need to monitor tissue repair in pre-clinical studies. To this end, we currently investigate optical coherence tomography, impedance sensing and their combination into organ-on-a chip technologies with a focus on ophthalmological applications.
We currently have projects on:
- Optical coherence tomography for tissue engineering and regenerative medicine
- Fourier-Domain optical coherence tomography
- Optical coherence microscopy
- Noninvasive biosensors and quantitative methods in medicine and biology
- Electric cell-substrate impedance sensing (ECIS, http://www.biophysics.com/)
- Common-path optical coherence phase microscopy
– live cell optical fluctuations
Pierre Bagnaninchi is a member of EPSRC, BBSRC and MRC funded Organ on a chip technologies network (www.organonachip.org.uk), for which he leads a Special interest group: Label-free real-time monitoring, translation to organ on a chip.